Coding

Part:BBa_K2933128:Design

Designed by: Wenhui Gong   Group: iGEM19_TJUSLS_China   (2019-09-14)


His+Linker a+Sumo+Linker b+CPS-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 256
    Illegal PstI site found at 892
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 256
    Illegal NheI site found at 33
    Illegal PstI site found at 892
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 256
    Illegal BglII site found at 145
    Illegal BglII site found at 1174
    Illegal BamHI site found at 344
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 256
    Illegal PstI site found at 892
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 256
    Illegal PstI site found at 892
    Illegal AgeI site found at 793
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized in E. coli BL21(DE3).



Source

NCBI

References